Cell Guidance Systems

protein

PODS™ Human EGF

PPH26-50

50 million

145 USD

PPH26-50

PODS™ Human EGF

growth Factors

50 million

145 USD

Epidermal Growth Factor (EGF) is a Growth factor that stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells. EGF contains three intramolecular disulfide bonds and binds in hiGh affinity to the epidermal Growth factor receptor (EGFR). EGF is overexpressed in multiple tumor cell lines and promotes resistance to chemotherapy and radiation treatments.

Human

PODS™ crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biolo gical activity of 50 million PODS™ crystals generates the same peak dose as 3.3 μ g of standard recombinant protein. However, at 5 days followin g the start of seedin g the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we su g gest usin g 50 million PODS™ crystals in place of 3.3 μ g of standard growth factor as a startin g point." To control for cross-reactivity with cells or as a ne gative control, we recommend usin g PODS™ growth factors alon gside PODS™ Empty crystals, as the latter do not contain or release car go protein.

Upon receipt, store at 4°C. PODS™ protein crystals are stable for at least 1 year when dry and 6 months when resuspended.