protein

PODS™ Human GDNF

PPH2-50

50 million

145 USD

PODS™ Human GDNF

growth Factors

50 million

145 USD

Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that siGnals throuGh a multicomponent receptor system to activate receptor tyrosine kinase RET siGnalinG. GDNF promotes dopamine uptake, prevents motor neuron apoptosis, and supports the survival and differentiation of neurons.

Human

PODS™ crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biolo gical activity of 50 million PODS™ crystals generates the same peak dose as 3.3 μ g of standard recombinant protein. However, at 5 days followin g the start of seedin g the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we su g gest usin g 50 million PODS™ crystals in place of 3.3 μ g of standard growth factor as a startin g point." To control for cross-reactivity with cells or as a ne gative control, we recommend usin g PODS™ growth factors alon gside PODS™ Empty crystals, as the latter do not contain or release car go protein.

Upon receipt, store at 4°C. PODS™ protein crystals are stable for at least 1 year when dry and 6 months when resuspended.