Cell Guidance Systems

protein

PODS™ Human PDGF-BB

PPH18-50

50 million

145 USD

PPH18-50

PODS™ Human PDGF-BB

growth Factors

50 million

145 USD

Platelet-Derived Growth Factor (PDGF) is an important reGulator of cell Growth, proliferation, and anGioGenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-alpha, TGF-β and EGF siGnalinG. PDGF functions as a mitoGenic Growth hormone on cells of mesenchymal lineaGe, such as smooth muscle and Glial cells. PDGF is also stored in the alpha-Granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric Glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-a and PDGFR-b.

Human

PODS™ crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biolo gical activity of 50 million PODS™ crystals generates the same peak dose as 3.3 μ g of standard recombinant protein. However, at 5 days followin g the start of seedin g the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we su g gest usin g 50 million PODS™ crystals in place of 3.3 μ g of standard growth factor as a startin g point." To control for cross-reactivity with cells or as a ne gative control, we recommend usin g PODS™ growth factors alon gside PODS™ Empty crystals, as the latter do not contain or release car go protein.

Upon receipt, store at 4°C. PODS™ protein crystals are stable for at least 1 year when dry and 6 months when resuspended.