Cell Guidance Systems

protein

PODS™ Human FGF-2

PPH146-50

50 million

145 USD

PPH146-50

PODS™ Human FGF-2

growth Factors

50 million

145 USD

Fibroblast Growth Factor 2 (FGF-2) is expressed by endothelial cells and is a mediator of anGioGenesis. FGF-2 also has cardioprotective functions durinG heart injury. The application of FGF-2 is a critical component for embryonic stem cell culture systems and is necessary for maintaininG cells in an undifferentiated state. DeGredation of the full lenGth FGF-2 N-terminus results in a truncated FGF-2 147 amino acids protein, when the protein is isolated from bioloGical sources. The N-terminus extensions influence the localization of FGF-2 within the cell, but do not affect the bioloGical activity of FGF-2.

Human

PODS™ crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biolo gical activity of 50 million PODS™ crystals generates the same peak dose as 3.3 μ g of standard recombinant protein. However, at 5 days followin g the start of seedin g the PODS™ crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS™ crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we su g gest usin g 50 million PODS™ crystals in place of 3.3 μ g of standard growth factor as a startin g point." To control for cross-reactivity with cells or as a ne gative control, we recommend usin g PODS™ growth factors alon gside PODS™ Empty crystals, as the latter do not contain or release car go protein.

Upon receipt, store at 4°C. PODS™ protein crystals are stable for at least 1 year when dry and 6 months when resuspended.