product summary
company name :
Briar Patch Biosciences
product type :
antibody
product name :
Protamine
catalog :
MAb-Hup2B-150
quantity :
150 µg
price :
275 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Hup2B
reactivity :
human
application :
immunohistochemistry, chromatin immunoprecipitation
citations: 12
Reference
Hammadeh M, Hamad M, Montenarh M, Fischer-Hammadeh C. Protamine contents and P1/P2 ratio in human spermatozoa from smokers and non-smokers. Hum Reprod. 2010;25:2708-20 pubmed publisher
Li W, Wu J, Kim S, Zhao M, Hearn S, Zhang M, et al. Chd5 orchestrates chromatin remodelling during sperm development. Nat Commun. 2014;5:3812 pubmed publisher
Hammoud S, Liu L, Carrell D. Protamine ratio and the level of histone retention in sperm selected from a density gradient preparation. Andrologia. 2009;41:88-94 pubmed publisher
Tanaka H, Iguchi N, Isotani A, Kitamura K, Toyama Y, Matsuoka Y, et al. HANP1/H1T2, a novel histone H1-like protein involved in nuclear formation and sperm fertility. Mol Cell Biol. 2005;25:7107-19 pubmed
Zhao M, Shirley C, Mounsey S, Meistrich M. Nucleoprotein transitions during spermiogenesis in mice with transition nuclear protein Tnp1 and Tnp2 mutations. Biol Reprod. 2004;71:1016-25 pubmed
Yan W, Ma L, Burns K, Matzuk M. HILS1 is a spermatid-specific linker histone H1-like protein implicated in chromatin remodeling during mammalian spermiogenesis. Proc Natl Acad Sci U S A. 2003;100:10546-51 pubmed
Zhong J, Peters A, Lee K, Braun R. A double-stranded RNA binding protein required for activation of repressed messages in mammalian germ cells. Nat Genet. 1999;22:171-4 pubmed
van der Heijden G, Dieker J, Derijck A, Muller S, Berden J, Braat D, et al. Asymmetry in histone H3 variants and lysine methylation between paternal and maternal chromatin of the early mouse zygote. Mech Dev. 2005;122:1008-22 pubmed
Zhao M, Shirley C, Hayashi S, Marcon L, Mohapatra B, Suganuma R, et al. Transition nuclear proteins are required for normal chromatin condensation and functional sperm development. Genesis. 2004;38:200-13 pubmed
van der Heijden G, Derijck A, Ramos L, Giele M, van der Vlag J, de Boer P. Transmission of modified nucleosomes from the mouse male germline to the zygote and subsequent remodeling of paternal chromatin. Dev Biol. 2006;298:458-69 pubmed
Cho C, Willis W, Goulding E, Jung-Ha H, Choi Y, Hecht N, et al. Haploinsufficiency of protamine-1 or -2 causes infertility in mice. Nat Genet. 2001;28:82-6 pubmed
Kimura T, Ito C, Watanabe S, Takahashi T, Ikawa M, Yomogida K, et al. Mouse germ cell-less as an essential component for nuclear integrity. Mol Cell Biol. 2003;23:1304-15 pubmed
product information
Catalog Number :
MAb-Hup2B-150
Product Name :
Hup2B
Web URL Link :
www.briarpatchbio.com
Product Type :
Primary antibody
Host Species :
Mouse
Clonality :
Monoclonal
Conjugation :
Unconjugated
Clone Name :
Hup2B
Isotype :
IgG1
Light Chain :
Unknown
Size :
150 µg
List Price :
275 USD
list of Pubmed id :
15922569, 20823111, 19260844, 16887113, 16055721, 24818823, 15083521, 15163613, 12920187, 12556490, 11326282, 10369260
Image Legend :
Anti-protamine antibody
Product Description :
Monoclonal antibody recognizing protamine P2
CrossReactivity :
Most mammalian protamine P2
SpeciesSummary :
Protamine P2 (precursor and processed forms) from human, mouse, Syrian hamster, rat
Immunogen :
Human protamine
Epitope :
RRRLHRIHRRQH
Localization :
Sperm and spermatids
Specificity :
Mammalian protamines
Purity :
Not determined
Buffer :
PBS, pH 7.4
Packaging :
Polypropylene tube
Concentration :
2.1 mg/ml
Uses :
Mammalian protamine P2 detection and quantification
Application Summary :
Western blots, IHC, CHIP
Storage :
'-20°C long term, 4 to 6°C for weeks
company information
Briar Patch Biosciences
15986 Mines Road, Livermore, CA 94550
www.briarpatchbio.com
1-925-203-5657
headquarters: USA