GDNF Glial-Derived Neurotrophic Factor Rat Recombinant Protein

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product summary

company name :

Boster

product type :

protein

product name :

GDNF Glial-Derived Neurotrophic Factor Rat Recombinant Protein

catalog :

PROTQ07731

quantity :

2 ug, 10 ug, 1 mg

product information

SKU :

PROTQ07731

Product Name :

GDNF Glial-Derived Neurotrophic Factor Rat Recombinant Protein

Product Page URL :

https://www.bosterbio.comgdnf-glial-derived-neurotrophic-factor-rat-recombinant-protein-protq07731-boster.html

Gene Name :

Gdnf

Size :

2 ug, 10 ug, 1 mg

Reactivity :

Mouse

Applications :

Cell Culture

Introduction :

Introduction: GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Description :

Glial derived Neurotrophic Factor Rat Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 134 amino acids and having a total molecular mass of 29.8 kDa.

Source :

E. Coli

Form :

Sterile Filtered White lyophilized (freeze-dried) powder.

Contents :

GDNF was lyophilized from a sterile solution containing 1xPBS, pH 7.4.

AA Sequence and/or Biological Activity :

Amino Acid Sequence:SPDKQAALP RRENRNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCESAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDNLVY HILRKHSAKR CGCI Biological Activity:Recombinant rat GDNF has full biological activity when compared to standards. The ED50, determined by a cell proliferation assay using rat C6 cells, is less than 0.2ng/ml corresponding to a specific activity of more than 5,000,000IU/mg.

Purification :

Greater than 98.0% as determined by HPLC analysis and by SDS-PAGE.

Storage :

Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Uniprot ID :

Q07731

Gene Full Name :

Glial cell line-derived neurotrophic factor

Synonyms :

Glial cell line-derived neurotrophic factor;Astrocyte-derived trophic factor;ATF;Gdnf;

Protein Function :

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. May also modulate local neuronal effects in distal regions of the motor neuron.

Subcellular Localization :

Secreted.

Tissue Specificity :

Expressed in both the central nervous system (CNS) and in non-CNS tissues, including the kidney, lung, bone, heart, liver, spleen, sciatic nerve and blood. In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Localizes at the proximal ligature of the hypoglossal nerve.