Eotaxin Mouse Recombinant Protein (CCL11) | Boster PROTP48298 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

protein

product name :

Eotaxin Mouse Recombinant Protein (CCL11)

catalog :

PROTP48298

quantity :

5 ug, 20 ug, 1 mg

product information

SKU :

PROTP48298

Product Name :

Eotaxin Mouse Recombinant Protein (CCL11)

Product Page URL :

https://www.bosterbio.comeotaxin-mouse-recombinant-protein-ccl11-protp48298-boster.html

Gene Name :

Ccl11

Size :

5 ug, 20 ug, 1 mg

Reactivity :

Mouse

Applications :

Cell Culture

Introduction :

Introduction: Chemokine (C-C motif) ligand 11 (CCL11) is a small cytokine belonging to the CC chemokine family that is also known as eotaxin. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. The effects of CCL11 are mediated by its binding to a G-protein-linked receptor known as a chemokine receptor. Chemokine receptors for which CCL11 is a ligand include CCR2, CCR3 and CCR5. The gene for human CCL11 (scya11) is encoded on three exons and is located on chromosome 17.

Description :

Eotaxin Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8403.2 Dalton. The CCL11 is purified by proprietary chromatographic techniques.

Source :

E. Coli

Form :

Sterile Filtered White lyophilized (freeze-dried) powder.

Contents :

Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

AA Sequence and/or Biological Activity :

Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be His-Pro-Gly-Ser-Ile. Biological Activity: The Biological activity was determined by measuring the dose dependent phosphorylation of ERK1 and ERK2 in CCR3 transfected 293 cells. Significant ERK phosphorylation is observed with >100 ng/ml (corresponding to a Specific Activity of 10,000IU/mg) of recombinant mouse eotaxin.

Purification :

Greater than 95.0% as determined by:. (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Storage :

Lyophilized Eotaxin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Uniprot ID :

P48298

Gene Full Name :

Eotaxin

Synonyms :

Eotaxin;C-C motif chemokine 11;Eosinophil chemotactic protein;Small-inducible cytokine A11;Ccl11;Scya11;

Protein Function :

In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils.

Subcellular Localization :

Secreted.

Tissue Specificity :

Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney.