GDNF Glial-Derived Neurotrophic Factor Human Recombinant Protein

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product summary

company name :

Boster

product type :

protein

product name :

GDNF Glial-Derived Neurotrophic Factor Human Recombinant Protein

catalog :

PROTP39905

quantity :

2 ug, 10 ug, 1 mg

product information

SKU :

PROTP39905

Product Name :

GDNF Glial-Derived Neurotrophic Factor Human Recombinant Protein

Product Page URL :

https://www.bosterbio.comgdnf-glial-derived-neurotrophic-factor-human-recombinant-protein-protp39905-boster.html

Gene Name :

GDNF

Size :

2 ug, 10 ug, 1 mg

Reactivity :

Mouse

Applications :

Cell Culture

Introduction :

Introduction: GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Description :

Glial derived Neurotrophic Factor Human Recombinant produced in E.Coli is a non-glycosylated disulfide-linked homodimer containing 2 x 135 amino acids and having a total molecular mass of approximately 30kDa. GDNF is purified by proprietary chromatographic techniques.

Source :

E. Coli

Form :

Sterile Filtered White lyophilized (freeze-dried) powder.

AA Sequence and/or Biological Activity :

Amino Acid Sequence: MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI.

Purification :

Greater than 95.0% as determined by:. (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Storage :

Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Uniprot ID :

P39905

Gene Full Name :

Glial cell line-derived neurotrophic factor

Synonyms :

Glial cell line-derived neurotrophic factor;hGDNF;Astrocyte-derived trophic factor;ATF;GDNF;

Protein Function :

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Subcellular Localization :

Secreted

Tissue Specificity :

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.