protein

MCP-1 Monocyte Chemotactic Protein-1 Rat Recombinant Protein (CCL2)

PROTP14844

2 ug, 10 ug, 1 mg

MCP-1 Monocyte Chemotactic Protein-1 Rat Recombinant Protein (CCL2)

https://www.bosterbio.commcp-1-monocyte-chemotactic-protein-1-rat-recombinant-protein-ccl2-protp14844-boster.html

Ccl2

2 ug, 10 ug, 1 mg

Mouse

Cell Culture

Introduction: Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor ?B (NF?B). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weightof approximately 13kDa. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5.

Monocyte Chemotactic Protein-1 Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 125 amino acids and having a molecular mass of 14.1 kDa. The MCP-1 is purified by proprietary chromatographic techniques.

E. Coli

Sterile Filtered White lyophilized (freeze-dried) powder.

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Amino Acid Sequence:QPDAVNAPLT CCYSFTGKMI PMSRLENYKR ITSSRCPKEA VVFVTKLKRE ICADPNKEWV QKYIRKLDQN QVRSETTVFY KIASTLRTSA PLNVNLTHKS EANASTLFST TTSSTSVEVT SMTEN. Biological Activity:ED50 =1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg. The biological activity was determined by measuring the dose dependent chemotaxis with human THP-1 cells. The optimal concentration should be determined for each specific application by an initial dose-response assay.

Greater than 98.0% as determined by SDS-PAGE.

Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL2 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

P14844

C-C motif chemokine 2

C-C motif chemokine 2;Immediate-early serum-responsive protein JE;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;MCP-1;Small-inducible cytokine A2;Ccl2;Je, Mcp1, Scya2;

Chemotactic factor that attracts monocytes, but not neutrophils.

Secreted.