Anti-BCA1/CXCL13 Antibody Picoband™ | Boster PB9999 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-BCA1/CXCL13 Antibody Picoband™

catalog :

PB9999

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry

product information

SKU :

PB9999

Product Name :

Anti-BCA1/CXCL13 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Mouse

Application(s) :

ELISA, IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Mouse, By Heat. ELISA, 0.1-0.5µg/ml, Mouse Western blot, 0.1-0.5µg/ml, Mouse.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-BCA1/CXCL13 Antibody Picoband™ catalog # PB9999. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

Cxcl13

Uniprot ID :

O55038

Immunogen :

E. coli-derived mouse BCA1 recombinant protein (Position: I22-A109). Mouse BCA1 shares 45.3% amino acid (aa) sequence identity with human BCA1.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

C-X-C motif chemokine 13

Synonyms :

C-X-C motif chemokine 13; B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; Cxcl13; Blc, Scyb13;

Protein Name :

C-X-C motif chemokine 13

Molecular Weight :

11927 MW

Protein Function :

Strongly chemotactic for B-lymphocytes, weakly for spleen monocytes and macrophages but no chemotactic activity for granulocytes. Binds to BLR1/CXCR5. May play a role in directing the migration of B-lymphocytes to follicles in secondary lymphoid organs.

Subcellular Localization :

Secreted.

Tissue Specificity :

Found in spleen (B-cell-rich zone or follicles), Peyer patches (strongest within germinal centers and extending to the mantle zone) and lymph nodes (in reticular pattern in follicles).

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

C-X-C motif chemokine 13 (CXCL13), also known as B cell-attracting chemokine 1 (BCA-1), is a protein that in humans is encoded by the CXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family. Its gene is mapped to human chromosome 4 in a cluster of other CXC chemokines. This protein has contained 109 amino acids. It is expressed highly in the liver, spleen, lymph nodes and gut of humans. The protein induces chemotaxis of BLR1-expressing B cells.