Anti-EAAT1/SLC1A3 Antibody Picoband™ | Boster PB9959 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-EAAT1/SLC1A3 Antibody Picoband™

catalog :

PB9959

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

SKU :

PB9959

Product Name :

Anti-EAAT1/SLC1A3 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Mouse, Rat.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-EAAT1/SLC1A3 Antibody Picoband™ catalog # PB9959. Tested in WB applications. This antibody reacts with Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

SLC1A3

Uniprot ID :

P43003

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human EAAT1 (14-42aa RMERFQQGVRKRTLLAKKKVQNITKEDVK), different from the related mouse sequence by three amino acids, and identical to the related rat sequence.

Form :

Lyophilized

Contents :

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Excitatory amino acid transporter 1

Synonyms :

Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3; SLC1A3; EAAT1, GLAST, GLAST1;

Protein Name :

Excitatory amino acid transporter 1

Molecular Weight :

59572 MW

Protein Function :

Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.

Subcellular Localization :

Membrane; Multi-pass membrane protein.

Tissue Specificity :

Highly expressed in cerebellum, but also found in frontal cortex, hippocampus and basal ganglia.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Background :

Solute carrier family 1 (glial high-affinity glutamate transporter), member 3, also known as SLC1A3, EAAT1 or GLAST, is a protein that in humans is encoded by the SLC1A3 gene. This gene is a member of high affinity glutamate transporter family. It is mapped to chromosome 5p13.2 by fluorescence in situ hybridization (FISH). And this gene transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Furthermore, this gene acts as a symport by cotransporting sodium.

Research Category :

Amino Acids, Cell Type Marker, Energy Metabolism, Metabolism, Neural Stem Cells, Neuroscience, Neurotransmitter, Signal Transduction, Stem Cells, Surface Molecules, Transporters