Anti-MEF2A Antibody Picoband™ | Boster PB9953 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-MEF2A Antibody Picoband™

catalog :

PB9953

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

product information

SKU :

PB9953

Product Name :

Anti-MEF2A Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

IF, IHC-P, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Rat. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Immunocytochemistry/Immunofluorescence, 2µg/ml, Human.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-MEF2A Antibody Picoband™ catalog # PB9953. Tested in IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

MEF2A

Uniprot ID :

Q02078

Immunogen :

VT), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
DGSDREDPRGDFHSPIVLGRPPNTEDRESPSVKRMRMDA
W
A synthetic peptide corresponding to a sequence at the C-terminus of human MEF2A (466-507aa

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Myocyte-specific enhancer factor 2A

Synonyms :

Myocyte-specific enhancer factor 2A; Serum response factor-like protein 1; MEF2A; MEF2;

Protein Name :

Myocyte-specific enhancer factor 2A

Molecular Weight :

54811 MW

Protein Function :

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT] (4)TAR-3', found in numerous muscle- specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.

Subcellular Localization :

Nucleus.

Tissue Specificity :

Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

Myocyte-specific enhancer factor 2A is a protein that in humans is encoded by the MEF2A gene. It is mapped to 15q26. The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.

Research Category :

Calcium Signaling, Calmodulin Pathway, Cardiogenesis, Cardiovascular, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Hypertrophy, Mesenchymal Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Signal Transduction, Signaling Pathway, Stem Cells, Transcription, Transcription Factors, Transcription Factors/Regulators