Anti-Calcium Sensing Receptor/CASR Antibody Picoband™

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Calcium Sensing Receptor/CASR Antibody Picoband™

catalog :

PB9924

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

PB9924

Product Name :

Anti-Calcium Sensing Receptor/CASR Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Mouse, Rat, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Calcium Sensing Receptor/CASR Antibody Picoband™ catalog # PB9924. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

CASR

Uniprot ID :

P41180

Immunogen :

E. coli-derived human CASR recombinant protein (Position: Q926-S1078). Human CASR shares 80.5% and 78.6% amino acid (aa) sequence identity with mouse and rat CASR, respectively.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Extracellular calcium-sensing receptor

Synonyms :

Extracellular calcium-sensing receptor; CaSR; Parathyroid cell calcium-sensing receptor 1; PCaR1; CASR; GPRC2A, PCAR1;

Protein Name :

Extracellular calcium-sensing receptor

Molecular Weight :

120674 MW

Protein Function :

Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G- protein that activates a phosphatidylinositol-calcium second messenger system.

Subcellular Localization :

Cell membrane ; Multi-pass membrane protein.

Tissue Specificity :

Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

The calcium-sensing receptor (CaSR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism.

Research Category :

Calcium Signaling, Cancer, Cofactors, Vitamins / Minerals, Cytoskeleton / Ecm, Extracellular Matrix, G Protein Signaling, Growth Factors/Hormones, Lipid Signaling, Metabolism, Neuroscience, Neurotransmission, Pathways And Processes, Receptors / Channels, Signal Transduction, Signaling Pathway, Structures, Vitamins / Minerals