Anti-Fatty Acid Synthase/FASN Antibody Picoband™ | Boster PB9865 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Fatty Acid Synthase/FASN Antibody Picoband™

catalog :

PB9865

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

PB9865

Product Name :

Anti-Fatty Acid Synthase/FASN Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

IHC, ICC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, By Heat. Immunocytochemistry,0.5-1µg/ml Western blot, 0.1-0.5µg/ml.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Fatty Acid Synthase/FASN Antibody Picoband™ catalog # PB9865. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

FASN

Uniprot ID :

P49327

Immunogen :

E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Fatty acid synthase

Synonyms :

Fatty acid synthase; 2.3.1.85; [Acyl-carrier-protein] S-acetyltransferase; 2.3.1.38; [Acyl-carrier-protein] S-malonyltransferase; 2.3.1.39; 3-oxoacyl-[acyl-carrier-protein] synthase; 2.3.1.41; 3-oxoacyl-[acyl-carrier-protein] reductase; 1.1.1.100; 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; 4.2.1.59; Enoyl-[acyl-carrier-protein] reductase; 1.3.1.39; Oleoyl-[acyl-carrier-protein] hydrolase; 3.1.2.14; FASN; FAS;

Protein Name :

Fatty acid synthase

Molecular Weight :

273427 MW

Protein Function :

Fatty acid synthetase catalyzes the formation of long- chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

Subcellular Localization :

Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity :

Ubiquitous. Prominent expression in brain, lung, and liver.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

Fatty acid synthase (FAS) is an enzyme that in humans is encoded by the FASN gene. It is mapped to 17q25. The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Research Category :

Cancer, Cancer Metabolism, Cardiovascular, Energy Metabolism, Energy Transfer Pathways, Fatty Acid Oxidation, Fatty Acids, Integration Of Energy, Integration Of Energy Metabolism, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipids / Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Lipids And Lipoproteins, Neurology Process, Neuroscience, Obesity, Pathways And Processes, Redox Metabolism, Signal Transduction, Tumor Biomarkers