Anti-PKLR Antibody Picoband™ | Boster PB9499 product information

product summary

company name :

Boster

product type :

antibody

product name :

Anti-PKLR Antibody Picoband™

catalog :

PB9499

quantity :

100μg/vial

price :

315 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Boster product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; loading ...; fig 2b	Prater M, Hamilton R, Wa Yung H, Sharkey A, Robson P, Abd Hamid N, et al. RNA-Seq reveals changes in human placental metabolism, transport and endocrinology across the first-second trimester transition. Biol Open. 2021;10: pubmed publisher

image

image 1 :

Anti-PKLR Picoband antibody, PB9499, Western blotting. All lanes: Anti PKLR (PB9499) at 0.5ug/ml. Lane 1: Rat Liver Tissue Lysate at 50ug. Lane 2: Mouse Liver Tissue Lysate at 50ug. Predicted bind size: 62KD. Observed bind size: 62KD

image 2 :

Anti-PKLR Picoband antibody, PB9499, IHC(P). IHC(P): Human Intestinal Cancer Tissue

product information

SKU :

PB9499

Product Name :

Anti-PKLR Antibody Picoband™

Price :

315 USD

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Mouse, Rat, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-PKLR Antibody Picoband™ catalog # PB9499. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

PKLR

Uniprot ID :

P30613

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human PKLR (522-552aa EAIWADDVDRRVQFGIESGKLRGFLRVGDLV), different from the related mouse sequence by one amino acid, and identical to the rat sequence.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Pyruvate kinase PKLR

Synonyms :

Pyruvate kinase PKLR; 2.7.1.40; Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase; PKLR; PK1, PKL;

Protein Name :

Pyruvate kinase PKLR

Molecular Weight :

61830 MW

Protein Function :

Plays a key role in glycolysis.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the pyruvate kinase family.

Background :

Pyruvate kinase isozymes R/L is an enzyme that in humans is encoded by the PKLR gene. It is mapped to 1q21. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

Research Category :

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Pathways And Processes, Signal Transduction

more info or order :

Boster product webpage