Anti-Syndecan 4/SDC4 Antibody Picoband™ | Boster PB9388 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Syndecan 4/SDC4 Antibody Picoband™

catalog :

PB9388

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

PB9388

Product Name :

Anti-Syndecan 4/SDC4 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat .

Application Notes :

WB: The detection limit for Syndecan 4 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Syndecan 4/SDC4 Antibody Picoband™ catalog # PB9388. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

SDC4

Uniprot ID :

P31431

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Syndecan 4 (172-198aa MKKKDEGSYDLGKKPIYKKAPTNEFYA), identical to the related mouse and rat sequences.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Syndecan-4

Synonyms :

Syndecan-4; SYND4; Amphiglycan; Ryudocan core protein; SDC4;

Protein Name :

Syndecan-4

Molecular Weight :

21642 MW

Protein Function :

Cell surface proteoglycan that bears heparan sulfate.

Subcellular Localization :

Isoform 1: Membrane; Single-pass type I membrane protein. Secreted. Shedding of the ectodomain produces a soluble form.

Tissue Specificity :

Expressed in epithelial and fibroblastic cells.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

Syndecan 4 is also known as SDC4. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.

Research Category :

Cell Adhesion Proteins, Cytoskeleton / Ecm, Ecm Proteins, Neurogenesis, Neurology Process, Neuroscience, Plasma Membrane, Signal Transduction, Subcellular Markers, Tags & Cell Markers