Anti-Calcineurin A/PPP3CA Antibody Picoband™ | Boster PB9382 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Calcineurin A/PPP3CA Antibody Picoband™

catalog :

PB9382

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

SKU :

PB9382

Product Name :

Anti-Calcineurin A/PPP3CA Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Predicted Reactivity :

Bovine

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Calcineurin A/PPP3CA Antibody Picoband™ catalog # PB9382. Tested in WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

PPP3CA

Uniprot ID :

Q08209

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Calcineurin A (488-521aa DAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ), different from the related mouse and rat sequences by one amino acid.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Synonyms :

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; 3.1.3.16; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit alpha isoform; PPP3CA; CALNA, CNA;

Protein Name :

Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform

Molecular Weight :

58688 MW

Protein Function :

Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.

Subcellular Localization :

Cell membrane. Cell membrane, sarcolemma. Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the PPP phosphatase family. PP-2B subfamily.

Background :

Calcineurin A is also known as PPP3CA. It is mapped to 4q24. Semsarian et al. (1999) and Musaro et al. (1999) independently showed that IGF1stimulates skeletal muscle hypertrophy and a switch to glycolytic metabolism by activating calcineurin A and inducing the nuclear translocation of transcription factor NFATC1. Semsarian et al. (1999) found that hypertrophy was suppressed by the calcineurin inhibitors cyclosporin A or FK506, but not by inhibitors of the MAP kinase or phosphatidylinositol-3-OH kinase pathways. Musaro et al. (1999) showed that expression of a dominant-negative calcineurin mutant also repressed myocyte differentiation and hypertrophy. Musaro et al. (1999) demonstrated that either IGF1 or activated calcineurin induces expression of transcription factor GATA2, which accumulates in a subset of myocyte nuclei, where it associates with calcineurin and a specific dephosphorylated isoform of NFATC1.