Anti-Phospholipase D1/PLD1 Antibody Picoband™ | Boster PB9380 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Phospholipase D1/PLD1 Antibody Picoband™

catalog :

PB9380

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

product information

SKU :

PB9380

Product Name :

Anti-Phospholipase D1/PLD1 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse.

Application Notes :

WB: The detection limit for PLD1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Phospholipase D1/PLD1 Antibody Picoband™ catalog # PB9380. Tested in WB applications. This antibody reacts with Human, Mouse.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

PLD1

Uniprot ID :

Q13393

Immunogen :

E.coli-derived human PLD1 recombinant protein (Position: M1-H330). Human PLD1 shares 91.8% and 90.9% amino acid (aa) sequence identity with mouse and rat PLD1, respectively.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Phospholipase D1

Synonyms :

Phospholipase D1; PLD 1; hPLD1; 3.1.4.4; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1; PLD1;

Protein Name :

Phospholipase D1

Molecular Weight :

124184 MW

Protein Function :

Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).

Subcellular Localization :

Cytoplasm, perinuclear region. Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side. Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side. Late endosome membrane ; Lipid- anchor ; Cytoplasmic side.

Tissue Specificity :

Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the phospholipase D family.

Background :

By somatic cell hybrid analysis, PLD1 is mapped to 3q26.31. This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. And PLD1 disrupted association of gamma-secretase protein components, independent of PLD1catalytic activity. What's more, PLD1 regulates intracellular trafficking of beta-amyloid, distinct from its effect on gamma-secretase activity.

Research Category :

Cardiovascular, Lipases, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Lipids / Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Protein Trafficking, Signal Transduction, Signaling Pathway, Vesicle Transport