Anti-FRZB Antibody Picoband™ | Boster PB9372 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-FRZB Antibody Picoband™

catalog :

PB9372

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, ELISA

product information

SKU :

PB9372

Product Name :

Anti-FRZB Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

ELISA, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human ELISA, 0.1-0.5µg/ml, Human.

Application Notes :

WB: The detection limit for FRZB is approximately 0.1ng/lane under reducing conditions. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-FRZB Antibody Picoband™ catalog # PB9372. Tested in ELISA, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

FRZB

Uniprot ID :

Q92765

Immunogen :

E.coli-derived human FRZB recombinant protein (Position: E176-N325). Human FRZB shares 90% amino acid (aa) sequence identity with mouse FRZB.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Secreted frizzled-related protein 3

Synonyms :

Secreted frizzled-related protein 3; sFRP-3; Frezzled; Fritz; Frizzled-related protein 1; FrzB-1; FRZB; FIZ, FRE, FRP, FRZB1, SFRP3;

Protein Name :

Secreted frizzled-related protein 3

Molecular Weight :

36254 MW

Protein Function :

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.

Subcellular Localization :

Secreted.

Tissue Specificity :

Expressed primarily in the cartilaginous cores of the long bone during embryonic and fetal development and in the appendicular skeleton (6-13 weeks). At 13 weeks of gestation, transcripts were present in early chondroblasts of the tarsal bones of the foot, the carpal bones of the hands and the epiphysis of long bones. Highly expressed in placenta and heart, followed by brain, skeletal muscle, kidney and pancreas. Weakly expressed in lung and liver.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the secreted frizzled-related protein (sFRP) family.

Background :

FRZB is also known as FRE or OS1. The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. FRZB is a Wnt-binding protein especially important in embryonic development. It is a competitor for the cell-surface G-protein receptor Frizzled. FRZB is localized in the extracellular plasma membrane. Unlike frizzled, frzb lacks the 7 transmembrane domains normally found in G-protein-coupled receptors. It is still considered a homolog of frizzled because it contains a Cysteine Rich Domain (CRD), and because of its intracellular C-terminus which is crucial for signaling.

Research Category :

Cytoskeleton / Ecm, Extracellular Matrix, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures