Anti-SIRT6 Antibody Picoband™ | Boster PB9357 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-SIRT6 Antibody Picoband™

catalog :

PB9357

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

product information

SKU :

PB9357

Product Name :

Anti-SIRT6 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

WB: The detection limit for SIRT6 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-SIRT6 Antibody Picoband™ catalog # PB9357. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

SIRT6

Uniprot ID :

Q8N6T7

Immunogen :

E.coli-derived human SIRT6 recombinant protein (Position: D14-E180). Human SIRT6 shares 95% amino acid (aa) sequence identity with mouse SIRT6.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

NAD-dependent protein deacetylase sirtuin-6

Synonyms :

NAD-dependent protein deacetylase sirtuin-6; 3.5.1.-; Regulatory protein SIR2 homolog 6; SIR2-like protein 6; SIRT6; SIR2L6;

Protein Name :

NAD-dependent protein deacetylase sirtuin-6

Molecular Weight :

39119 MW

Protein Function :

NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF- kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD (+) in the absence of acetylated substrate.

Subcellular Localization :

Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the sirtuin family. Class IV subfamily.

Background :

SIRT6 is also known as SIR2L6. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.

Research Category :

Acetylation, Apoptosis, Atherosclerosis, Cancer, Cardiovascular, ChIP’ing Antibodies, Chromatin Modifying Enzymes, Diabetes Associated, Epigenetics and Nuclear Signaling, Metabolism, Nfkb Pathway, Nuclear Signaling Pathways, Obesity, Signal Transduction