Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband™

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband™

catalog :

PB9345

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

product information

SKU :

PB9345

Product Name :

Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse.

Application Notes :

WB: The detection limit for TPA is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband™ catalog # PB9345. Tested in WB applications. This antibody reacts with Human, Mouse.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

PLAT

Uniprot ID :

P00750

Immunogen :

E.coli-derived human TPA recombinant protein (Position: H366-P562). Human TPA shares 83% and 84% amino acid (aa) sequence identity with mouse and rat TPA, respectively.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Tissue-type plasminogen activator

Synonyms :

Tissue-type plasminogen activator; t-PA; t-plasminogen activator; tPA; 3.4.21.68; Alteplase; Reteplase; Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B; PLAT;

Protein Name :

Tissue-type plasminogen activator

Molecular Weight :

62917 MW

Protein Function :

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration.

Subcellular Localization :

Secreted, extracellular space.

Tissue Specificity :

Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the peptidase S1 family.

Background :

PLAT is also known as tPA. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.