Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband™

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband™

catalog :

PB9277

quantity :

100μg/vial

price :

315 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:500–1:750; fig s73 74 immunohistochemistry - paraffin section; human; 1:500–1:750; fig s73 74	Rickelt S, Hynes R. Antibodies and methods for immunohistochemistry of extracellular matrix proteins. Matrix Biol. 2018;71-72:10-27 pubmed publisher

image

image 1 :

Figure 1. Western blot analysis of HSPG2 using anti- HSPG2 antibody (PB9277). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, . Lane 2: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- HSPG2 antigen affinity purified polyclonal antibody (Catalog # PB9277) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPG2 at approximately 469KD. The expected band size for HSPG2 is at 469KD.

image 2 :

Anti-HSPG2 Picoband antibody, PB9277,IHC(P). IHC(P): Human Lung Cancer Tissue

product information

SKU :

PB9277

Product Name :

Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband™

Price :

315 USD

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

IHC-P, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

WB: The detection limit for HSPG2 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Heparan Sulfate Proteoglycan 2/HSPG2 Antibody Picoband™ catalog # PB9277. Tested in IHC-P, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

HSPG2

Uniprot ID :

P98160

Immunogen :

E.coli-derived human HSPG2 recombinant protein (Position: F524-K701). Human HSPG2 shares 86% amino acid (aa) sequence identity with mouse HSPG2.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Basement membrane-specific heparan sulfate proteoglycan core protein

Synonyms :

Basement membrane-specific heparan sulfate proteoglycan core protein; HSPG; Perlecan; PLC; Endorepellin; LG3 peptide; HSPG2;

Protein Name :

Basement membrane-specific heparan sulfate proteoglycan core protein

Molecular Weight :

468830 MW

Protein Function :

Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.

Subcellular Localization :

Secreted, extracellular space, extracellular matrix, basement membrane.

Tissue Specificity :

Found in the basement membranes.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Contains 4 EGF-like domains.

Background :

Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.

Research Category :

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cytoskeleton / Ecm, Extracellular Matrix, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Lipids And Lipoproteins, Pathways And Processes, Signal Transduction

more info or order :

Boster product webpage