Anti-Prostate Specific Antigen/KLK3 Antibody Picoband™

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Prostate Specific Antigen/KLK3 Antibody Picoband™

catalog :

PB9259

quantity :

100μg/vial

price :

315 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

Boster product webpage

citations: 1

Reference
Jin B, An H. Baicalin alleviates benign prostate hyperplasia through androgen-dependent apoptosis. Aging (Albany NY). 2020;12:2142-2155 pubmed publisher

image

image 1 :

Figure 1. Western blot analysis of KLK3 using anti-KLK3 antibody (PB9259). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLK3 antigen affinity purified polyclonal antibody (Catalog # PB9259) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KLK3 at approximately 29KD. The expected band size for KLK3 is at 29KD.

product information

SKU :

PB9259

Product Name :

Anti-Prostate Specific Antigen/KLK3 Antibody Picoband™

Price :

315 USD

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

IHC-P, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human. Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/ml, Human.

Application Notes :

WB: The detection limit for PSA is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Prostate Specific Antigen/KLK3 Antibody Picoband™ catalog # PB9259. Tested in IHC-P, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

KLK3

Uniprot ID :

P07288

Immunogen :

E.coli-derived human PSA recombinant protein (Position: I25-P261). Human PSA shares 54% amino acid (aa) sequence identity with rat PSA.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Prostate-specific antigen

Synonyms :

Prostate-specific antigen; PSA; 3.4.21.77; Gamma-seminoprotein; Seminin; Kallikrein-3; P-30 antigen; Semenogelase; KLK3; APS;

Protein Name :

Prostate-specific antigen

Molecular Weight :

28741 MW

Protein Function :

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Subcellular Localization :

Secreted.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti- Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the peptidase S1 family. Kallikrein subfamily.

Background :

Prostate-specific antigen (PSA), also known as gamma-seminoprotein or kallikrein-3 (KLK3), is a glycoprotein enzyme encoded in humans by the KLK3 gene. KLK3 is a member of the kallikrein-related peptidase family and is secreted by the epithelial cells of the prostate gland. This gene is mapped to 19q13.33. KLK3 is present in small quantities in the serum of men with healthy prostates, but is often elevated in the presence of prostate cancer or other prostate disorders. KLK3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely. It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm. It is not a unique indicator of prostate cancer, but may also detect prostatitis or benign prostatic hyperplasia.

Research Category :

Cancer, Cell Adhesion Proteins, Cell Type Markers, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Neuroscience, Signal Transduction, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens

more info or order :

Boster product webpage