antibody

Anti-Galectin 1/LGALS1 Picoband Antibody

PB9240

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Enabled

Anti-Galectin 1/LGALS1 Picoband Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

LGALS1

Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Polyclonal antibody for GALECTIN 1/LGALS1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. GALECTIN 1/LGALS1 information: Molecular Weight: 14716 MW; Subcellular Localization: Secreted, extracellular space, extracellular matrix ; Tissue Specificity: Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Rabbit IgG polyclonal antibody for Galectin-1(LGALS1) detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM in Human;Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

P09382

Rabbit

E.coli-derived human Galectin 1 recombinant protein (Position: A2-D135). Human Galectin 1 shares 88% and 90% amino acid (aa) sequences identity with mouse and rat Galectin 1, respectively.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunocytochemistry/Immunofluorescence , 2ug/ml, Human, Rat, -. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Human, -. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5ug/ml, Human, Rat. Flow Cytometry, 1-3ug/1x10 6 cells, Human.

Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Human, Mouse, Rat

Anti- Galectin1 antibody, PB9240, Western blotting. All lanes: Anti Galectin1(PB9240) at 0.5ug/ml. WB: Recombinant Human Galectin1 Protein 0.5ng. Predicted bind size: 16KD. Observed bind size: 16KD Anti- Galectin1 antibody, PB9240, Western blotting. All lanes: Anti Galectin1(PB9240) at 0.5ug/ml. Lane 1: Rat Testis Tissue Lysate at 50ug. Lane 2: Rat Brain Tissue Lysate at 50ug. Lane 3: Rat Liver Tissue Lysate at 50ug. Lane 4: Rat Kidney Tissue Lysate at 50ug. Lane 5: HELA Whole Cell Lysate at 40ug. Lane 6: SMMC Whole Cell Lysate at 40ug. Lane 7: NRK Whole Cell Lysate at 40ug. Predicted bind size: 15KD. Observed bind size: 15KD Anti- Galectin1 antibody, PB9240,IHC(P). IHC(P): Rat Cardiac Muscle Tissue Anti- Galectin1 antibody, PB9240,IHC(P). IHC(P): Mouse Cardiac Muscle Tissue Anti- Galectin1 antibody, PB9240,IHC(P). IHC(P): Human Placenta Tissue Anti- Galectin1 antibody, PB9240,IHC(F). IHC(F): Human Placenta Tissue Anti- Galectin1 antibody, PB9240,ICC. ICC: A549 Cell Figure 8. ICC analysis of Galectin 1 using anti- Galectin 1 antibody (PB9240). Galectin 1 was detected in immunocytochemical section of rat NRK cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Galectin 1 Antibody (PB9240) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 9. IHC analysis of Galectin 1 using anti- Galectin 1 antibody (PB9240). Galectin 1 was detected in frozen section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Galectin 1 Antibody (PB9240) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 10. IF analysis of Galectin 1 using anti- Galectin 1 antibody (PB9240) . Galectin 1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/mL rabbit anti- Galectin 1 Antibody (PB9240) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Figure 11. Flow Cytometry analysis of U20S cells using anti- Galectin 1 antibody (PB9240). Overlay histogram showing U20S cells stained with PB9240 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- Galectin 1 Antibody (PB9240,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.

sc 166617 sc 166618 sc 166619 sc 19276 sc 19277 sc 28248 sc 31793

Cell Biology

Galectin-1;Gal-1;14 kDa laminin-binding protein;HLBP14;14 kDa lectin;Beta-galactoside-binding lectin L-14-I;Galaptin;HBL;HPL;Lactose-binding lectin 1;Lectin galactoside-binding soluble 1;Putative MAPK-activating protein PM12;S-Lac lectin 1;LGALS1;

Galectin-1

14716 MW

May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.

Secreted, extracellular space, extracellular matrix .

Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.

Galectin-1

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Contains 1 galectin domain.

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