Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband™ | Boster PB9212 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband™

catalog :

PB9212

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunohistochemistry - paraffin section

product information

SKU :

PB9212

Product Name :

Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

IHC-P, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human. Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human.

Application Notes :

WB: The detection limit for HMOX1 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband™ catalog # PB9212. Tested in IHC-P, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

HMOX1

Uniprot ID :

P09601

Immunogen :

E.coli-derived human HMOX1 recombinant protein (Position: M1-M288). Human HMOX1 shares 82% and 80% amino acid (aa) sequences identity with mouse and rat HMOX1, respectively.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Heme oxygenase 1

Synonyms :

Heme oxygenase 1; HO-1; 1.14.99.3; HMOX1; HO, HO1;

Protein Name :

Heme oxygenase 1

Molecular Weight :

32819 MW

Protein Function :

Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Subcellular Localization :

Microsome. Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side.

Tissue Specificity :

Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level).

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the heme oxygenase family.

Background :

HMOX1 (heme oxygenase (decycling) 1), also known as HO-1, is a human gene that encodes for the enzyme heme oxygenase 1. It is an essential enzyme in heme catabolism, it cleaves heme to form biliverdin. HMOX1 belongs to the heme oxygenase family. The HMOX1 gene is located on the long (q) arm of chromosome 22 at position 12.3, from base pair 34,101,636 to base pair 34,114,748. HMOX1, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. HMOX1 activity is induced by its substrate heme and by various nonheme substances.

Research Category :

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Epigenetics and Nuclear Signaling, Heart Disease, Metabolism, Metabolism Processes, Neurodegenerative Disease, Neurology Process, Neuroscience, Nfkb Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Pathways And Processes, Platelets, Response To Hypoxia, Signal Transduction, Signaling Pathway, Vascular Inflammation, Vasculature