Anti-FOXO3A Antibody Picoband™ | Boster PB9196 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-FOXO3A Antibody Picoband™

catalog :

PB9196

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunohistochemistry

product information

SKU :

PB9196

Product Name :

Anti-FOXO3A Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-FOXO3A Antibody Picoband™ catalog # PB9196. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

FOXO3

Uniprot ID :

O43524

Immunogen :

E.coli-derived human FOXO3A recombinant protein (Position: Q471-G673). Human FOXO3A shares 97% amino acid (aa) sequence identity with mouse FOXO3A.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Forkhead box protein O3

Synonyms :

Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3; FKHRL1, FOXO3A;

Protein Name :

Forkhead box protein O3

Molecular Weight :

71277 MW

Protein Function :

Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Subcellular Localization :

Cytoplasm, cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors.

Tissue Specificity :

Ubiquitous.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Contains 1 fork-head DNA-binding domain.

Background :

Forkhead box O3, also known as FKHRL1 or FOXO3a, is a human protein encoded by the FOXO3 gene. FOXO3 belongs to the O subclass of the forkhead family of transcription factors which are characterized by a distinct fork head DNA-binding domain. It is mapped to 6q21. This protein likely functions as a trigger for apoptosis through upregulation of genes necessary for cell death, such as Bim and PUMA, or downregulation of anti-apoptotic proteins such as FLIP. In mammals FOXO3 regulates the resistance of cells to stress by inducing DNA repair and thereby may also affect organismal life span. In addition, it is thought that FOXO3 is also involved in protection from oxidative stress by upregulating antioxidants such as catalase and MnSOD.

Research Category :

Co-Activators/Co-Repressors, Domain Families, Epigenetics and Nuclear Signaling, Forkhead Box, Hypoxia, Intracellular, Metabolism, Metabolism Processes, Nuclear Receptors, Nuclear Signaling Pathways, Oncoproteins, Oncoproteins/Suppressors, Pathways And Processes, Transcription, Transcription Factors