Anti-FGFR4 Antibody Picoband™ | Boster PB9193 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-FGFR4 Antibody Picoband™

catalog :

PB9193

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry

product information

SKU :

PB9193

Product Name :

Anti-FGFR4 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

WB: The detection limit for FGFR4 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-FGFR4 Antibody Picoband™ catalog # PB9193. Tested in IHC, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

FGFR4

Uniprot ID :

P22455

Immunogen :

E.coli-derived human FGFR4 recombinant protein (Position: L22-H206). Human FGFR4 shares 86% and 84% amino acid (aa) sequences identity with mouse and rat FGFR4, respectively.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Fibroblast growth factor receptor 4

Synonyms :

Fibroblast growth factor receptor 4; FGFR-4; 2.7.10.1; CD334; FGFR4; JTK2, TKF;

Protein Name :

Fibroblast growth factor receptor 4

Molecular Weight :

87954 MW

Protein Function :

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.

Subcellular Localization :

Cell membrane; Single-pass type I membrane protein. Endosome. Endoplasmic reticulum. Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane.

Tissue Specificity :

Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.

Background :

FGFR4 (Fibroblast growth factor receptor 4), also known as CD334, is a protein that in humans is encoded by the FGFR4 gene. It is mapped to 5q35.2. The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. It is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis.

Research Category :

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cell Type Marker, Cell Type Markers, Growth Factors, Growth Factors/Hormones, Neural Stem Cells, Neuroscience, Signal Transduction, Stem Cells, Surface Molecules, Tags & Cell Markers