Anti-NFkB p100/p52/NFKB2 MYD88 Antibody Picoband™ | Boster PB9150 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-NFkB p100/p52/NFKB2 MYD88 Antibody Picoband™

catalog :

PB9150

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

PB9150

Product Name :

Anti-NFkB p100/p52/NFKB2 MYD88 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Bovine

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

WB: The detection limit for NFkB p100/p52 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-NFkB p100/p52/NFKB2 MYD88 Antibody Picoband™ catalog # PB9150. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

NFKB2

Uniprot ID :

Q99836

Immunogen :

E.coli-derived human NFkB p100/p52 recombinant protein (Position: M1-R340). Human NFkB p100/p52 shares 96% amino acid (aa) sequence identity with mouse NFkB p100/p52.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Myeloid differentiation primary response protein MyD88

Synonyms :

Myeloid differentiation primary response protein MyD88; MYD88;

Protein Name :

Nuclear factor NF-kappa-B p100 subunit

Molecular Weight :

33233 MW

Protein Function :

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.

Subcellular Localization :

Cytoplasm

Tissue Specificity :

Ubiquitous.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Contains 7 ANK repeats.

Background :

NFKB2, also known as nuclear factor NF-kappa-B p100 subunit, is a protein that in humans is encoded by the NFKB2 gene. It is mapped to 10q24.32. This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFKB). NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner.

Research Category :

Adapters, Atherosclerosis, Cardiovascular, Cytoplasmic, Immunology, Innate Immunity, Signal Transduction, Tlr Signaling, Vascular Inflammation