Anti-BTK Picoband Antibody | Boster PB9134 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-BTK Picoband Antibody

catalog :

PB9134

quantity :

100 ug/vial

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

product information

sku :

PB9134

name :

Anti-BTK Picoband Antibody

price :

240 USD

immunogen :

E.coli-derived human BTK recombinant protein (Position: A2-P340). Human BTK shares 98% amino acid (aa) sequence identity with mouse BTK.

form :

Lyophilized

host :

Rabbit

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

purification :

Immunogen affinity purified.

clonality :

Polyclonal

description :

Rabbit IgG polyclonal antibody for Tyrosine-protein kinase BTK(BTK) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

size :

100 ug/vial

reactivity :

Human; Mouse; Rat

applications :

IHC-P; WB

background :

BTK, also known as Bruton's tyrosine kinase, is an enzyme that in humans is encoded by the BTK gene. It is mapped to Xq22.1. BTK plays a crucial role in B cell maturation as well as mast cell activation through the high-affinity IgE receptor. BTK contains a PH domain that binds phosphatidylinositol (3,4,5)-trisphosphate (PIP3). PIP3 binding induces BTK to phosphorylate phospholipase C, which in turn hydrolyzes PIP2, a phosphatidylinositol, into two second messengers, inositol triphosphate (IP3) and diacylglycerol (DAG), which then go on to modulate the activity of downstream proteins during B-cell signalling. This gene also regulates both TLR9 activation and expression in B lymphocytes and is necessary for inhibitory cytokine expression.

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

cross reactivity :

No cross reactivity with other proteins

gene full name :

Tyrosine-protein kinase BTK

gene name :

BTK

predicted reactivity :

Hamster

protein function :

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.

protein name :

Tyrosine-protein kinase BTK

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

research category :

signal transduction protein phosphorylation tyrosine kinases immunology innate immunity tlr signaling

sequence similarities :

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

storage :

At -20ËšC for one year. After reconstitution, at 4ËšC for one month. It can also be aliquotted and stored frozen at -20ËšC for a longer time.Avoid repeated freezing and thawing.

subcellular localization :

Cytoplasm. Cell membrane; Peripheral membrane protein. Nucleus. In steady state, BTK is predominantly cytosolic. Following B-cell receptor (BCR) engagement by antigen, translocates to the plasma membrane through its PH domain. Plasma membrane localization is a critical step in the activation of BTK. A fraction of BTK also shuttles between the nucleus and the cytoplasm, and nuclear export is mediated by the nuclear export receptor CRM1.

synonyms :

Tyrosine-protein kinase BTK;2.7.10.2;Agammaglobulinemia tyrosine kinase;ATK;B-cell progenitor kinase;BPK;Bruton tyrosine kinase;BTK;AGMX1, ATK, BPK;

tissue specificity :

Predominantly expressed in B-lymphocytes.

uniprot id :

Q06187