Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody | Boster PB9096-Dyl488 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody

catalog :

PB9096-Dyl488

quantity :

50 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

DyLight488

reactivity :

human

application :

immunocytochemistry, flow cytometry

product information

SKU :

PB9096-Dyl488

Product Name :

Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody

Size :

50 ug/vial

Clonality :

Polyclonal

Host :

Rabbit

Conjugate :

DyLight®488

Reactivity :

Human

Application(s) :

Flow Cytometry, IF

Application Details :

Immunofluorescence, 2µg/ml, Human. Flow Cytometry, 1-3μg/1x10^6 cells, Human.

Application Notes :

Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Human CD45 DyLight® 488 conjugated PTPRC Antibody catalog # PB9096-Dyl488. Tested in Flow Cytometry, IF applications. This antibody reacts with Human.

Gene Name :

PTPRC

Uniprot ID :

P08575

Immunogen :

E.coli-derived human CD45 recombinant protein (Position: R1113-S1304). Human CD45 shares 68% amino acid (aa) sequence identity with both mouse and rat CD45.

Form :

Liquid

Contents :

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

At 2-8?C for one year from date of receipt. Protect from light. Do not freeze.

Gene Full Name :

Receptor-type tyrosine-protein phosphatase C

Synonyms :

Receptor-type tyrosine-protein phosphatase C; 3.1.3.48; Leukocyte common antigen; L-CA; T200; CD45; PTPRC; CD45;

Protein Name :

Receptor-type tyrosine-protein phosphatase C

Molecular Weight :

147254 MW

Protein Function :

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

Subcellular Localization :

Membrane ; Single-pass type I membrane protein. Membrane raft. Colocalized with DPP4 in membrane rafts.

Sequence Similarities :

Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.

Background :

CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene. CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase. The cytogenetic location of CD45 is 1q31.3-q32.1. This gene is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.

Research Category :

Adapters, Adaptive Immunity, B Cells, Cell Type Markers, Immunology, Protein Phosphorylation, Signal Transduction, T Cells, Transmembrane, Tyrosine Phosphatases