Anti-Von Willebrand Factor/VWF Antibody Picoband™ | Boster PB9062 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Von Willebrand Factor/VWF Antibody Picoband™

catalog :

PB9062

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

product information

SKU :

PB9062

Product Name :

Anti-Von Willebrand Factor/VWF Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

Flow Cytometry, IHC-P, IHC-F, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml. Immunocytochemistry, 0.5-1µg/ml. Flow Cytometry, 1-3μg/1x10^6 cells.

Application Notes :

WB: The detection limit for VWF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Von Willebrand Factor/VWF Antibody Picoband™ catalog # PB9062. Tested in Flow Cytometry, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

VWF

Uniprot ID :

P04275

Immunogen :

E.coli-derived human VWF recombinant protein (Position: R2535-K2813). Human VWF shares 79% amino acid (aa) sequence identity with mouse VWF.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

von Willebrand factor

Synonyms :

von Willebrand factor; vWF; von Willebrand antigen 2; von Willebrand antigen II; VWF; F8VWF;

Protein Name :

von Willebrand factor

Molecular Weight :

309265 MW

Protein Function :

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

Subcellular Localization :

Secreted. Secreted, extracellular space, extracellular matrix. Localized to storage granules.

Tissue Specificity :

Plasma

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Sequence Similarities :

Contains 1 CTCK (C-terminal cystine knot-like) domain.

Background :

Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.

Research Category :

Angiogenesis, Cancer, Cardiovascular, Invasion/Microenvironment, Platelets, Serum Proteins, Stem Cells