Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™ | Boster PB9002 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™

catalog :

PB9002

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

PB9002

Product Name :

Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IHC-P, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat. Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human, Mouse, Rat, By Heat. Flow Cytometry, 1-3 μg/1x10^6 cells, Human.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-COX1/Cyclooxygenase 1/PTGS1 Antibody Picoband™ catalog # PB9002. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

PTGS1

Uniprot ID :

P23219

Immunogen :

E.coli-derived human COX1 recombinant protein (Position: E318-L599). Human COX1 shares 90% and 89% amino acid (aa) sequences identity with mouse and rat COX1, respectively.

Form :

Lyophilized

Contents :

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Prostaglandin G/H synthase 1

Synonyms :

Prostaglandin G/H synthase 1; 1.14.99.1; Cyclooxygenase-1; COX-1; Prostaglandin H2 synthase 1; PGH synthase 1; PGHS-1; PHS 1; Prostaglandin-endoperoxide synthase 1; PTGS1; COX1;

Protein Name :

Prostaglandin G/H synthase 1

Molecular Weight :

68686 MW

Protein Function :

Converts arachidonate to prostaglandin H2 (PGH2), a committed step in prostanoid synthesis. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.

Subcellular Localization :

Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the prostaglandin G/H synthase family.

Background :

Cyclooxygenase 1 (COX1), also known as Prostaglandin-endoperoxide synthase (PTGS1) or mitochondrial cytochrome c oxidase subunit 1, is the key enzyme in prostaglandin biosynthesis. The gene was approximately 40 kb long, with 11 protein-coding exons. There were 599 amino acid residues with a calculated molecular mass of approximately 68 kD. By analysis of a human/hamster somatic hybrid DNA panel, it was demonstrated that the PTGS1 gene maps to chromosome 9. Human prostaglandin endoperoxide synthase exhibited 91% amino acid identity with the sheep enzyme. Prostaglandin synthase 1 gene disruption in mice reduces arachidonic acid-induced inflammation and indomethacin-induced gastric ulceration.

Research Category :

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Endocrine Metabolism, Heart Disease, Hormone Biosynthesis, Immunology, Innate Immunity, Lipid And Lipoprotein Metabolism, Lipid Signaling, Macrophage / Inflamm., Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Oxidative Stress, Pathways And Processes, Platelets, Redox Metabolism, Signal Transduction, Signaling Pathway