antibody

Anti-TAB1 Antibody

PA2288

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

Enabled

Anti-TAB1 Antibody

Primary Antibodies, Polyclonal Antibodies

TAB1

30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Polyclonal antibody for TAB1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. TAB1 information: Molecular Weight: 54644 MW; Tissue Specificity: Ubiquitous.

Rabbit IgG polyclonal antibody for TGF-beta-activated kinase 1/MAP3K7-binding protein 1(TAB1) detection. Tested with WB in Human;Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

Q15750

Rabbit

A synthetic peptide corresponding to a sequence at the C-terminus of human TAB1(304-321aa QANQEIAAMIDTEFAKQT), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat.

WB

Human, Mouse, Rat

Chicken

Figure 1. Western blot analysis of TAB1 using anti-TAB1 antibody (PA2288). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA Whole Cell Lysate . Lane 2: Mouse Brain Tissue Lysate . Lane 3: Mouse Testis Tissue Lysate . Lane 4: Mouse Spleen Tissue Lysate . Lane 5: HEPA Whole Cell Lysate . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB1 antigen affinity purified polyclonal antibody (Catalog # PA2288) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB1 at approximately 55KD. The expected band size for TAB1 is at 55KD. Figure 2. Western blot analysis of TAB1 using anti-TAB1 antibody (PA2288). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB1 antigen affinity purified polyclonal antibody (Catalog # PA2288) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB1 at approximately 55KD. The expected band size for TAB1 is at 55KD.

Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, also called TAB1, is an enzyme that in humans is encoded by the MAP3K7IP1 gene. It is mapped to 22q13.1. The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways. This protein can interact with and activate the mitogen-activated protein kinase 14(MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. TAB1 also can activate the kinase activity of TAK1 by direct interaction, and it may be an important signaling intermediate between TGFB receptors and TAK1. What's more, TAB1 plays an important role in skin homeostasis, wound repair, and oncogenesis.

sc 6052 sc 13956 sc 6053 sc 100869 sc 166138

Atherosclerosis, Cardiovascular, Immunology, Innate Immunity, Mapk Pathway, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction, Tlr Signaling, Vascular Inflammation

TGF-beta-activated kinase 1 and MAP3K7-binding protein 1;Mitogen-activated protein kinase kinase kinase 7-interacting protein 1;TGF-beta-activated kinase 1-binding protein 1;TAK1-binding protein 1;TAB1;MAP3K7IP1;

TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

54644 MW

May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.

Ubiquitous.

TGF-beta-activated kinase 1/MAP3K7-binding protein 1

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Contains 1 PPM-type phosphatase domain.

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