antibody

Anti-Apg7/ATG7 Antibody

PA2261

100μg/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

Anti-Apg7/ATG7 Antibody

100μg/vial

Polyclonal

Rabbit

Human, Mouse, Rat

Chicken

WB

Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Apg7/ATG7 Antibody catalog # PA2261. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

ATG7

O95352

A synthetic peptide corresponding to a sequence at the C-terminus of human Apg7 (652-669aa KVLDQYEREGFNFLAKVF), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

Lyophilized

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Ubiquitin-like modifier-activating enzyme ATG7

Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; hAGP7; Ubiquitin-activating enzyme E1-like protein; ATG7; APG7L;

Ubiquitin-like modifier-activating enzyme ATG7

77960 MW

E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.

Cytoplasm. Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Autophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. It is mapped to 3p25.3. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, ATG7 can regulate p53-dependent cell cycle and cell death pathways.

Cell Death, Energy Metabolism, Energy Transfer Pathways, Intracellular, Metabolic Signaling Pathways, Metabolism, Metabolism Processes, Mitochondrial Metabolism, Pathways And Processes, Proteasome / Ubiquitin, Proteolysis / Ubiquitin, Signal Transduction, Ub-Like Proteins