Anti-Collagen I/COL1A1 Antibody | Boster PA2140-2 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Collagen I/COL1A1 Antibody

catalog :

PA2140-2

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

product information

SKU :

PA2140-2

Product Name :

Anti-Collagen I/COL1A1 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

IF, IHC-P, IHC-F, ICC, WB

Application Details :

Immunofluorescence, 2μg/ml, HumanImmunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Immunocytochemistry, 0.5-1µg/ml, Mouse, Human, RatImmunohistochemistry (Frozen Section), 0.5-1µg/ml, Human, Rat, Mouse Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Application Notes :

WB: The detection limit for COL1A1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Collagen I/COL1A1 Antibody catalog # PA2140-2. Tested in IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

COL1A1

Uniprot ID :

P11087

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1192-1207aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Collagen alpha-1(I) chain

Synonyms :

Collagen alpha-1 (I) chain; Alpha-1 type I collagen; Col1a1; Cola1;

Protein Name :

Collagen alpha-1(I) chain

Molecular Weight :

138032 MW

Protein Function :

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Subcellular Localization :

Secreted, extracellular space, extracellular matrix.

Tissue Specificity :

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Sequence Similarities :

Belongs to the fibrillar collagen family.

Background :

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Research Category :

Collagen, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Mesenchymal Stem Cells, Signal Transduction, Stem Cells