Anti-Smad1 Antibody | Boster PA2114 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Smad1 Antibody

catalog :

PA2114

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

product information

SKU :

PA2114

Product Name :

Anti-Smad1 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Hamster

Application(s) :

IHC, ICC, WB

Application Details :

Immunocytochemistry , 0.5-1µg/ml, HumanImmunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.

Application Notes :

WB: The detection limit for SMAD1 is approximately 30ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Smad1 Antibody catalog # PA2114. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

SMAD1

Uniprot ID :

P70340

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Smad1 (253-267aa AEISRGDVQAVAYEE), different from the related rat sequence by one amino acid, and different from the related human sequence by two amino acids.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Mothers against decapentaplegic homolog 1

Synonyms :

Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; Dwarfin-A; Dwf-A; Mothers-against-DPP-related 1; Mad-related protein 1; mMad1; SMAD family member 1; SMAD 1; Smad1; Smad1; Madh1, Madr1;

Protein Name :

Mothers against decapentaplegic homolog 1

Molecular Weight :

52157 MW

Protein Function :

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) (By similarity). May play a role in the initiation and maintenance of spermatogenesis. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 (By similarity). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343).

Subcellular Localization :

Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4. Colocalizes with LEMD3 at the nucleus inner membrane (By similarity).

Tissue Specificity :

Ubiquitous.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Sequence Similarities :

Belongs to the dwarfin/SMAD family.

Background :

Mothers against decapentaplegic homolog 1 also known as SMAD family member 1 or SMAD1 is a protein that in humans is encoded by the SMAD1 gene. SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene "mothers against decapentaplegic" (Mad) and the C. elegans gene Sma. The gene was assigned to human chromosome 4q31.21. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.

Research Category :

Cancer, Cancer Metabolism, Cytoplasmic, Epigenetics and Nuclear Signaling, Growth Factors, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Pathways And Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, Tgf Beta