Anti-PC1/3/PCSK1 Antibody | Boster PA2084 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-PC1/3/PCSK1 Antibody

catalog :

PA2084

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunohistochemistry - paraffin section

product information

SKU :

PA2084

Product Name :

Anti-PC1/3/PCSK1 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Chicken

Application(s) :

IF, IHC-P, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat, . Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Immunofluorescence, 2µg/ml, Human

Application Notes :

WB: The detection limit for PCSK1 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-PC1/3/PCSK1 Antibody catalog # PA2084. Tested in IF, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

PCSK1

Uniprot ID :

P29120

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human PC1/3 (726-740aa DVFYNTKPYKHRDDR), identical to the related mouse and rat sequences.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Neuroendocrine convertase 1

Synonyms :

Neuroendocrine convertase 1; NEC 1; 3.4.21.93; Prohormone convertase 1; Proprotein convertase 1; PC1; PCSK1; NEC1;

Protein Name :

Neuroendocrine convertase 1

Molecular Weight :

84152 MW

Protein Function :

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

Subcellular Localization :

Cytoplasmic vesicle, secretory vesicle. Localized in the secretion granules.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the peptidase S8 family. Furin subfamily.

Background :

PCSK1 (Proprotein Convertase, Subtilisin/Kexin-Type, 1), also known as PC1 or NEC1, is an enzyme that in humans is encoded by the PCSK1 gene. Proprotein convertase-1 is a neuroendocrine convertase that belongs to a family of subtilisin-like serine endoproteases that process large precursor proteins into mature bioactive products. By in situ hybridization, Seidah et al. (1991) mapped the NEC1 gene to human chromosome 5q15-q21 and mouse chromosome 13. Ohagi et al. (1996) noted that PC1 initiates the sequential processing of proinsulin to insulin by cleaving the proinsulin molecule on the C-terminal side of the dibasic peptide, arg31-arg32, joining the B-chain and C-peptide. By observing the phenotypic features in patients with PC1 mutations, Jackson et al. (2003) concluded that human intestinal absorptive function is dependent on PC1 activity.

Research Category :

Cancer, Cancer Metabolism, Cell Biology, Endocrine Metabolism, Growth Factors/Hormones, Hormone Biosynthesis, Metabolic Signaling Pathway, Metabolism, Pathways And Processes, Proteolysis / Ubiquitin, Proteolytic Enzymes, Signal Transduction