Anti-Factor VII/F7 Antibody | Boster PA2060 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Factor VII/F7 Antibody

catalog :

PA2060

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

SKU :

PA2060

Product Name :

Anti-Factor VII/F7 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

WB: The detection limit for F7 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Factor VII/F7 Antibody catalog # PA2060. Tested in WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

Uniprot ID :

P08709

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Factor VII (441-453aa SQYIEWLQKLMRS).

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Coagulation factor VII

Synonyms :

Coagulation factor VII; 3.4.21.21; Proconvertin; Serum prothrombin conversion accelerator; SPCA; Eptacog alfa; Factor VII light chain; Factor VII heavy chain; F7;

Protein Name :

Coagulation factor VII

Molecular Weight :

51594 MW

Protein Function :

Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.

Subcellular Localization :

Secreted.

Tissue Specificity :

Plasma

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the peptidase S1 family.

Background :

F7 (Coagulation Factor VII), also known as proconvertin, is one of the proteins that causes blood to clot in the coagulation cascade. It is an enzyme of the serine protease class. The F7 gene maps to chromosome 13q34 (Millar et al., 2000). Synthesis of factors VII and X, as well as factors II and IX, takes place in the liver and requires vitamin K. Structural homologies of these factors, which are precursors of serine proteases, have been shown (Zur and Nemerson, 1981). Di Bitondo et al. (2002) used reporter gene analysis to show that inclusion of promoter regions of F7 reduced transcription activity in the presence of estrogenic factors. The effect was independent of promoter polymorphic haplotype.

Research Category :

Cell Type Markers, Class I, Cytoskeleton, Cytoskeleton / Ecm, Intermediate Filaments, Signal Transduction, Tags & Cell Markers, Tumor Associated