Anti-IKK beta/IKBKB Antibody | Boster PA2036 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-IKK beta/IKBKB Antibody

catalog :

PA2036

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

sku :

PA2036

status :

Enabled

name :

Anti-IKK beta/IKBKB Antibody

category name :

Primary Antibodies, Polyclonal Antibodies

gene name :

IKBKB

price various sizes :

30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240

clonality :

Polyclonal

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

conjugate :

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

description :

Polyclonal antibody for IKK beta/IKBKB detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. IKK beta/IKBKB information: Molecular Weight: 86564 MW; Subcellular Localization: Cytoplasm. Nucleus. Membrane raft. Colocalized with DPP4 in membrane rafts; Tissue Specificity: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.

short description :

Rabbit IgG polyclonal antibody for Inhibitor of nuclear factor kappa-B kinase subunit beta(IKBKB) detection. Tested with WB in Human.

size :

100 ug/vial

sample size available :

30ug for $99, contact us for details

uniprot id :

O14920

host :

Rabbit

immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human IKK beta(735-750aa TALDWSWLQTEEEEHS).

form :

Lyophilized

purification :

Immunogen affinity purified.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

cross reactivity :

No cross reactivity with other proteins

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

application details :

Western blot, 0.1-0.5ug/ml, Human.

applications :

reactivity :

Human

image labels :

Anti-IKK beta antibody, PA2036, Western blotting. All lanes: Anti IKK beta (PA2036) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: MCF-7 Whole Cell Lysate at 40ug. Lane 3: HT1080 Whole Cell Lysate at 40ug. Lane 4: SKOV Whole Cell Lysate at 40ug. Lane 5: M231 Whole Cell Lysate at 40ug. Lane 6: MCF-7 Whole Cell Lysate at 40ug. Predicted bind size: 87KD. Observed bind size: 87KD

background :

IKBKB(Inhibitor of Kappa Light Chain Gene Enhancer in B Cells, Kinase of, Beta), also known as IKKB or NFKBIKB , is a protein that in humans is encoded by the IKBKB gene. Shindo et al.(1998) mapped the IKBKB gene to chromosome 8p12-p11 by FISH. Mercurio et al.(1997) found that mutations in IKK2 had a more pronounced effect upon NFKB activation than did comparable mutations in IKK1. Yin et al.(1998) tested the activity of various antiinflammatory agents on the IKK complex. They demonstrated that aspirin and sodium salicylate specifically inhibit IKK-beta activity in vitro and in vivo by binding to IKK-beta to reduce ATP binding.

competitor equivalent skus :

sc 130152 sc 135948 sc 161731 sc 161733 sc 21660 sc 21660 R sc 21661 sc 21661 R sc 23470 R sc 271782 sc 34673 sc 34674 sc 56918 sc 7218 sc 7329 sc 7329 R sc 7330 sc 7607 sc 8014

research category :

Atherosclerosis, Cardiovascular, Epigenetics And Nuclear Signaling, Immunology, Innate Immunity, Nfkb Pathway, Nuclear Signaling Pathways, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction, Tlr Signaling, Vascular Inflammation

synonyms :

Inhibitor of nuclear factor kappa-B kinase subunit beta;I-kappa-B-kinase beta;IKK-B;IKK-beta;IkBKB;2.7.11.10;I-kappa-B kinase 2;IKK2;Nuclear factor NF-kappa-B inhibitor kinase beta;NFKBIKB;IKBKB;IKKB;

gene full name :

Inhibitor of nuclear factor kappa-B kinase subunit beta

molecular weight :

86564 MW

protein function :

Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.

subcellular localization :

Cytoplasm. Nucleus. Membrane raft. Colocalized with DPP4 in membrane rafts.

tissue specificity :

Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.

protein name :

Inhibitor of nuclear factor kappa-B kinase subunit beta

recommended detection systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

sequence similarities :

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

last modified :

1/28/19 19:39