Anti-Flotillin 2/FLOT2 Antibody | Boster PA2034 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Flotillin 2/FLOT2 Antibody

catalog :

PA2034

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

SKU :

PA2034

Product Name :

Anti-Flotillin 2/FLOT2 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Rat, Mouse, Human

Predicted Reactivity :

Hamster

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Application Notes :

WB: The detection limit for FLOT2 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Flotillin 2/FLOT2 Antibody catalog # PA2034. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

FLOT2

Uniprot ID :

Q14254

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Flotillin 2 (117-131aa TLTVEQIYQDRDQFA), identical to the related rat and mouse sequences.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Flotillin-2

Synonyms :

Flotillin-2; Epidermal surface antigen; ESA; Membrane component chromosome 17 surface marker 1; FLOT2; ESA1, M17S1;

Protein Name :

Flotillin-2

Molecular Weight :

47064 MW

Protein Function :

May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.

Subcellular Localization :

Cell membrane ; Peripheral membrane protein. Membrane, caveola ; Peripheral membrane protein. Endosome. Membrane-associated protein of caveolae.

Tissue Specificity :

In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the band 7/mec-2 family. Flotillin subfamily.

Background :

FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.

Research Category :

Adapters, Coat Proteins, Neuroscience, Neurotransmission, Organelles, Protein Trafficking, Secretory Vesicles, Signal Transduction, Subcellular Markers, Tags & Cell Markers, Transmembrane, Vesicle Transport