antibody

Anti-Adiponectin/ADIPOQ Antibody

PA2014-1

100μg/vial

315 USD

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

Anti-Adiponectin/ADIPOQ Antibody

315 USD

100μg/vial

Polyclonal

Rabbit

Human, Mouse, Rat

Hamster

ELISA, IHC, WB

ELISA, 0.1-0.5µg/ml, HumanImmunohistochemistry (Frozen Section), 0.5-1µg/ml, Rat, Human, MouseImmunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

WB: The detection limit for ADIPOQ is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Adiponectin/ADIPOQ Antibody catalog # PA2014-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

ADIPOQ

Q15848

A synthetic peptide corresponding to a sequence at the C-terminus of human ADIPOQ (224-244aa LYADNDNDSTFTGFLLYHDTN), different from the related mouse and rat sequences by one amino acid.

Lyophilized

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Immunogen affinity purified.

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Adiponectin

Adiponectin; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; ADIPOQ; ACDC, ACRP30, APM1, GBP28;

Adiponectin

26414 MW

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

Secreted.

Synthesized exclusively by adipocytes and secreted into plasma.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).

Contains 1 C1q domain.

ADIPOQ (Adipocyte-, C1q-, and Collagen Domain-Containing), also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene. Using FISH, Das et al. (2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27. By RNase protection and Western blot analysis, Schaffler et al. (1999) showed that APM1 is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum. By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Adiponectin is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.

Atherosclerosis, Cancer, Cancer Metabolism, Cardiovascular, Diabetes, Diabetes Associated, Growth Factors/Hormones, Heart Disease, Lipids / Lipoproteins, Mesenchymal Stem Cells, Metabolism, Metabolism Processes, Neurology Process, Neuroscience, Obesity, Pathways And Processes, Response To Hypoxia, Signal Transduction, Stem Cells