antibody

Anti-SPHK1 Antibody

PA1996

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

Enabled

Anti-SPHK1 Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

SPHK1

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Polyclonal antibody for Sphingosine Kinase 1/SPHK1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB, IHC-P, IHC-F, ICC, FCM. Reactive species: Human. Sphingosine Kinase 1/SPHK1 information: Molecular Weight: 42518 MW; Subcellular Localization: Cytoplasm. Nucleus. Cell membrane. Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner; Tissue Specificity: Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.

Rabbit IgG polyclonal antibody for Sphingosine kinase 1(SPHK1) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse.

100 ug/vial

30ug for $99, contact us for details

Q9NYA1

Rabbit

A synthetic peptide corresponding to a sequence in the middle region of human SPHK1(117-133aa AASLNHYAGYEQVTNED), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot, 0.1-0.5ug/ml, Human, Mouse. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, By Heat. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Human. Immunocytochemistry , 0.5-1ug/ml, Human. Flow Cytometry, 1-3ug/1x10 6 cells, Human.

Flow Cytometry, IHC-P, IHC-F, ICC, WB

Human, Mouse

Anti-SPHK1 antibody, PA1996, Western blotting. Lane 1: RAJI Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: Hut Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: 293T Cell Lysate. Anti-SPHK1 antibody, PA1996, IHC(P). IHC(P): Human Placenta Tissue Anti-SPHK1 antibody, PA1996, ICC. ICC: HELA Cell Figure 4. Flow Cytometry analysis of U87 cells using anti-SPHK1 antibody (PA1996). Overlay histogram showing U87 cells stained with PA1996 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPHK1 Antibody (PA1996, 1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 5. Flow Cytometry analysis of U20S cells using anti-SPHK1 antibody (PA1996). Overlay histogram showing U20S cells stained with PA1996 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPHK1 Antibody (PA1996, 1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

sc 22702 sc 365401 sc 48825 sc 34528 sc 100441

Cancer, Cancer Metabolism, G Protein Signaling, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Lipids And Lipoproteins, Pathways And Processes, Signal Transduction, Signaling Pathway

Sphingosine kinase 1;SK 1;SPK 1;2.7.1.91;SPHK1;SPHK, SPK;

Sphingosine kinase 1

42518 MW

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Cytoplasm. Nucleus. Cell membrane. Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.

Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.

Sphingosine kinase 1

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

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