Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody

catalog :

PA1993

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunohistochemistry

product information

SKU :

PA1993

Product Name :

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Rat

Predicted Reactivity :

Bovine

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Mitochondrial Pyruvate dehydrogenase kinase 1/PDK1 Antibody catalog # PA1993. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

PDK1

Uniprot ID :

Q15118

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1 (408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

Synonyms :

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; 2.7.11.2; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1; PDK1; PDHK1;

Protein Name :

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

Molecular Weight :

49244 MW

Protein Function :

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

Subcellular Localization :

Mitochondrion matrix.

Tissue Specificity :

Expressed predominantly in the heart. Detected at lower levels in liver, skeletal muscle and pancreas.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Sequence Similarities :

Belongs to the PDK/BCKDK protein kinase family.

Background :

PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al. (1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

Research Category :

Cancer, Cancer Metabolism, Carbohydrate Metabolism, Chromatin Modifying Enzymes, Energy Metabolism, Energy Transfer Pathways, Epigenetics and Nuclear Signaling, Integration Of Energy, Integration Of Energy Metabolism, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Methylation, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oncoproteins/Suppressors, Pathways And Processes, Signal Transduction, Tumor Suppressors