Anti-Caspase-3 (P17) Antibody | Boster PA1961 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Caspase-3 (P17) Antibody

catalog :

PA1961

quantity :

100 ug/vial

price :

200 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

sku :

PA1961

name :

Anti-Caspase-3 (P17) Antibody

price :

200 USD

immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-3(P17)(70-90aa DAANLRETFRNLKYEVRNKND).

form :

Lyophilized

host :

Rabbit

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

purification :

Immunogen affinity purified.

clonality :

Polyclonal

description :

Rabbit IgG polyclonal antibody for Caspase-3(CASP3) detection. Tested with WB in Human.

size :

100 ug/vial

reactivity :

Human

applications :

background :

CASP3(Caspase3 Apoptosis-Related Cysteine Protease), also known as YAMA, CPP32 or APOPAIN, is a caspase protein that interacts with caspase 8 and caspase 9. It is encoded by the CASP3 gene. The CASP3 protein is a member of the cysteine-aspartic acid protease(caspase) family. Tiso et al.(1996) used radiation hybrid mapping to localize the CPP32 gene to human chromosome 4q33-q35.1. Fernandes-Alnemri et al.(1994) found that overexpression of CPP32 in insect cells induced apoptosis. Coexpression of the 2 CPP32 subunits in insect cells also resulted in apoptosis. Tewari et al.(1995) showed that purified human ICE cleaved the Yama proenzyme into a proteolytically active form and that activated Yama cleaved PARP into the 85-kD apoptotic form.

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

cross reactivity :

No cross reactivity with other proteins

gene full name :

Caspase-3

gene name :

CASP3

predicted reactivity :

Hamster

protein function :

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

protein name :

Caspase-3

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

research category :

neuroscience neurology process neurodegenerative disease alzheimer's disease cell biology apoptosis intracellular caspases etc caspases neuroscience cancer invasion/microenvironment cell death apoptotic markers

sequence similarities :

Belongs to the peptidase C14A family.

storage :

At -20ËšC for one year. After reconstitution, at 4ËšC for one month. It can also be aliquotted and stored frozen at -20ËšC for a longer time.Avoid repeated freezing and thawing.

subcellular localization :

Cytoplasm

synonyms :

Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1;Caspase-3 subunit p17;Caspase-3 subunit p12;CASP3;CPP32;

tissue specificity :

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

uniprot id :

P42574