antibody

Anti-Smad4 Antibody

PA1953

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Enabled

Anti-Smad4 Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

SMAD4

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Polyclonal antibody for SMAD4 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. SMAD4 information: Molecular Weight: 60439 MW; Subcellular Localization: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD. PDPK1 prevents its nuclear translocation in response to TGF- beta.

Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 4(SMAD4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

Q13485

Rabbit

A synthetic peptide corresponding to a sequence at the N-terminus of human Smad4(97-113aa RLWRWPDLHKNELKHVK), identical to the related rat and mouse sequences.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5ug/ml, Human, Rat, Mouse.

IHC, WB

Human, Mouse, Rat

Chicken

Anti-Smad4 antibody, PA1953, Western blotting. Lane 1: Rat Skeletal Muscle Tissue Lysate. Lane 2: A549 Cell Lysate. Lane 3: U87 Cell Lysate. Anti-Smad4 antibody, PA1953, IHC(P). IHC(P): Human Lung Cancer Tissue Anti-Smad4 antibody, PA1953, IHC(P). IHC(P): Rat Intestine Tissue Anti-Smad4 antibody, PA1953, IHC(P). IHC(P): Rat Intestine Tissue Lysate Anti-Smad4 antibody, PA1953, IHC(P). IHC(P): Rat Brain Tissue Lysate

SMAD4(Mothers Against Decapentaplegic Drosophila Homolog of 4), also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene. It belongs to the Darfwin family of proteins that modulate members of the TGFbeta protein superfamily. Hahn et al.(1996) identified the SMAD4 gene on chromosome 18q21.1. Howe et al.(1998) identified the SMAD4 gene within a region on 18q21.1 defined by linkage analysis in a kindred with juvenile polyposis syndrome. To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands. SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.

sc 7966 sc 56479 sc 56775 sc 1908 sc 7154 sc 73599 sc 1909 sc 73040 sc 1909 R sc 7966 X sc 1908 X sc 7154 X sc 1909 X

Apoptosis, Cancer, Cancer Metabolism, Cardiogenesis, Cardiovascular, Cytoplasmic, Epigenetics And Nuclear Signaling, Hypertrophy, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Oncoproteins/Suppressors, Pathways And Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, Tgf Beta, Transcription Factors, Transcription Factors/Regulators, Tumor Suppressors, Vasculature

Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;Deletion target in pancreatic carcinoma 4;SMAD family member 4;SMAD 4;Smad4;hSMAD4;SMAD4;DPC4, MADH4;

Mothers against decapentaplegic homolog 4

60439 MW

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD. PDPK1 prevents its nuclear translocation in response to TGF- beta.

Mothers against decapentaplegic homolog 4

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the dwarfin/SMAD family.

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