Anti-Cytochrome P450 1A2/CYP1A2 Antibody | Boster PA1907 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Cytochrome P450 1A2/CYP1A2 Antibody

catalog :

PA1907

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

sku :

PA1907

status :

Enabled

name :

Anti-Cytochrome P450 1A2/CYP1A2 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

CYP1A2

price various sizes :

30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

clonality :

Polyclonal

concentration :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

conjugate :

contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

description :

Polyclonal antibody for CYTOCHROME P450 1A2/CYP1A2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. CYTOCHROME P450 1A2/CYP1A2 information: Molecular Weight: 58294 MW; Subcellular Localization: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein; Tissue Specificity: Liver.

short description :

Rabbit IgG polyclonal antibody for Cytochrome P450 1A2(CYP1A2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

size :

100 ug/vial

sample size available :

30ug for $99, contact us for details

uniprot id :

P05177

host :

Rabbit

immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human CYP1A2 (265-282aa QKTVQEHYQDFDKNSVRD), different from the related rat and mouse sequences by three amino acids.

form :

Lyophilized

purification :

Immunogen affinity purified.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

cross reactivity :

No cross reactivity with other proteins

reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

application details :

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Rat, Human, Mouse, By Heat . Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat .

applications :

IHC, WB

reactivity :

Human, Mouse, Rat

image labels :

Anti-CYP1A2 antibody, PA1907, Western blotting. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Liver Tissue Lysate. Lane 3: SMMC Cell Lysate. Lane 4: HEPA Cell Lysate. Anti-CYP1A2 antibody, PA1907, IHC(P). IHC(P): Rat Liver Tissue

background :

CYP1A2(Cytochrome P450, Subfamily I, Polypeptide 2) is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body. CYP1A2 is a member of the cytochrome P450 superfamily of enzymes. In humans, the CYP1A2 enzyme is encoded by the CYP1A2 gene. CYP1A2 localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons(PAHs), some of which are found in cigarette smoke. The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin. Ikeya et al.(1989) found that the human CYP1A2 gene spans almost 7.8 kb and contains 7 exons. The CYP1A2 gene is mapped on 15q24.1. CYP1A2 accounts for nearly 15% of the cytochrome P450 in the human liver. CYP1A2 displays higher activity in men than in women, and is inhibited by oral contraceptives. Inducers of CYP1A2 include cruciferous vegetables. Cigarette smoking has also been shown to increaseCYP1A2 activity. Expression of CYP1A2 appears to be induced by various dietary constituents.

competitor equivalent skus :

sc 53241 sc 73476 sc 9832 sc 30085 sc 9833 sc 53614 sc 393783 sc 9835 sc 9836 sc 514044

research category :

Cancer, Cancer Metabolism, Cardiovascular, Cytochromes, Drug Metabolism, Lipases, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipids / Lipoproteins, Metabolic Signaling Pathways, Metabolism, Mitochondrial Metabolism, Pathways And Processes, Signal Transduction

synonyms :

Cytochrome P450 1A2;1.14.14.1;CYPIA2;Cytochrome P(3)450;Cytochrome P450 4;Cytochrome P450-P3;CYP1A2;

gene full name :

Cytochrome P450 1A2

molecular weight :

58294 MW

protein function :

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. Participates in the bioactivation of carcinogenic aromatic and heterocyclic amines. Catalizes the N-hydroxylation of heterocyclic amines and the O- deethylation of phenacetin.

subcellular localization :

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

tissue specificity :

Liver

protein name :

Cytochrome P450 1A2

recommended detection systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

sequence similarities :

Belongs to the cytochrome P450 family.

last modified :

1/28/19 19:39