Anti-Krueppel-like factor 5 KLF5 Antibody | Boster PA1889 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Krueppel-like factor 5 KLF5 Antibody

catalog :

PA1889

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

SKU :

PA1889

Product Name :

Anti-Krueppel-like factor 5 KLF5 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Rat, Mouse, Human

Predicted Reactivity :

Hamster

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.

Application Notes :

WB: The detection limit for KLF5 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Krueppel-like factor 5 KLF5 Antibody catalog # PA1889. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

KLF5

Uniprot ID :

Q13887

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of human KLF5 (109-126aa HKKYRRDSASVVDQFFTD), identical to the related rat and mouse sequences.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Krueppel-like factor 5

Synonyms :

Krueppel-like factor 5; Basic transcription element-binding protein 2; BTE-binding protein 2; Colon krueppel-like factor; GC-box-binding protein 2; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; KLF5; BTEB2, CKLF, IKLF;

Protein Name :

Krueppel-like factor 5

Molecular Weight :

50792 MW

Protein Function :

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

Subcellular Localization :

Nucleus.

Tissue Specificity :

Expressed only in testis and placenta.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the krueppel C2H2-type zinc-finger protein family.

Background :

KLF5 (Krueppel-like factor 5) is a protein that in humans is encoded by the KLF5 gene. It is also known as BASIC TRANSCRIPTION ELEMENT-BINDING PROTEIN 2 or BTEB2. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Suske et al. (2005)stated that the human KLF5 gene maps to chromosome 13q21.33, and the mouse Klf5 gene to chromosome 14E2.1. Sogawa et al. (1993)isolated a cDNA encoding BTEB2. The predicted 219-amino acid protein contains 3 consecutive zinc finger motifs near the C terminus. The zinc finger domains were 59% and 64% identical to those of Sp1 (189906) and BTEB1, respectively. Both BTEB2 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding. The N-terminal region of BTEB2 is proline rich. Recombinant BTEB2 protein and Sp1 showed very similar DNA-binding specificity in vitro. When expressed in mammalian cells, BTEB2 activated the transcription of a reporter gene fused to a GC box-containing promoter. Sogawa et al. (1993) also identified the rat BTEB2 homolog. Northern blot analysis of rat tissues revealed that BTEB2 is expressed only in the testis and placenta.

Research Category :

Apoptosis, Cancer, Cancer Susceptibility, Cardiovascular, Cell Biology, Domain Families, Epigenetics and Nuclear Signaling, Hypertrophy, Intracellular, Krueppel Like Factor, Mesenchymal Stem Cells, Oncoproteins, Oncoproteins/Suppressors, P53 Pathway, Proto-Oncogenes, Stem Cells, Transcription, Transcription Factors