Anti-ErbB 3/ERBB3 Antibody | Boster PA1880 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-ErbB 3/ERBB3 Antibody

catalog :

PA1880

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

SKU :

PA1880

Product Name :

Anti-ErbB 3/ERBB3 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Predicted Reactivity :

Bovine

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

WB: The detection limit for ERBB3 is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-ErbB 3/ERBB3 Antibody catalog # PA1880. Tested in WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

ERBB3

Uniprot ID :

P21860

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 3 (162-181aa DWRDIVRDRDAEIVVKDNGR).

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Receptor tyrosine-protein kinase erbB-3

Synonyms :

Receptor tyrosine-protein kinase erbB-3; 2.7.10.1; Proto-oncogene-like protein c-ErbB-3; Tyrosine kinase-type cell surface receptor HER3; ERBB3; HER3;

Protein Name :

Receptor tyrosine-protein kinase erbB-3

Molecular Weight :

148098 MW

Protein Function :

Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5.

Subcellular Localization :

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Tissue Specificity :

Epithelial tissues and brain.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.

Background :

ERBB3 (V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3) also known as ONCOGENE ERBB3 or HER3, is an enzyme that in humans is encoded by the ERBB3 gene. This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. By in situ hybridization, Kraus et al. (1989) mapped the ERBB3 gene to chromosome 12q13. Carraway et al. (1994) demonstrated that ERBB3 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Most notably, EGFR and ErbB2 became markedly more promiscuous as the threshold was lowered, whereas ErbB3did not. Engelman et al. (2007) found that amplification of MET caused gefitinib resistance by driving ERBB3-dependent activation of phosphoinositide 3-kinase, a pathway though to be specific to EGFR/ERBB family receptors.

Research Category :

Apoptosis, Cell Biology, Developmental Biology, Growth Factors/Hormones, Intracellular, Kinases, Nervous System Development, Organogenesis, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases