Anti-Orai1 Antibody | Boster PA1857 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Orai1 Antibody

catalog :

PA1857

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

SKU :

PA1857

Product Name :

Anti-Orai1 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Bovine

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.

Application Notes :

WB: The detection limit for ORAI1 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Orai1 Antibody catalog # PA1857. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

ORAI1

Uniprot ID :

Q96D31

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Orai1 (278-301aa EFARLQDQLDHRGDHPLTPGSHYA), different from the related rat and mouse sequences by two amino acids.

Form :

Lyophilized

Contents :

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Calcium release-activated calcium channel protein 1

Synonyms :

Calcium release-activated calcium channel protein 1; Protein orai-1; Transmembrane protein 142A; ORAI1; CRACM1, TMEM142A;

Protein Name :

Calcium release-activated calcium channel protein 1

Molecular Weight :

32668 MW

Protein Function :

Ca (2+) release-activated Ca (2+) (CRAC) channel subunit which mediates Ca (2+) influx following depletion of intracellular Ca (2+) stores and channel activation by the Ca (2+) sensor, STIM1. CRAC channels are the main pathway for Ca (2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.

Subcellular Localization :

Cell membrane ; Multi-pass membrane protein. Cytoplasmic vesicle, autophagosome. Isoform beta is more mobile in the plasma membrane. Colocalizes with UBQLN1 in the autophagosome.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Sequence Similarities :

Belongs to the Orai family.

Background :

ORAI1 (ORAI calcium release-activated calcium modulator 1), also known as CRACM1, TMEM142A, Calcium release-activated calcium channel protein 1, Protein orai-1, Transmembrane protein 142A, FLJ14466, is a calcium selective ion channel that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes severe combined immunodeficiency (SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers. Prakriya et al. (2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion. Prakriya et al. (2006) showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.

Research Category :

Adaptive Immunity, Calcium Channels, Calcium Signaling, Immunology, Non-Cd, Signal Transduction, Signaling Pathway, T Cells