Anti-Cpn10/HSPE1 Antibody | Boster PA1790 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Cpn10/HSPE1 Antibody

catalog :

PA1790

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

PA1790

Product Name :

Anti-Cpn10/HSPE1 Antibody

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Predicted Reactivity :

Bovine

Application(s) :

Flow Cytometry, IF, IHC-P, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat. Immunocytochemistry , 0.5-1µg/ml, Human. Immunofluorescence, 2μg/ml, Human. Immunocytochemistry/Immunofluorescence, 5µg/ml, Human. Flow Cytometry, 1-3μg/1x10^6 cells, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Cpn10/HSPE1 Antibody catalog # PA1790. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

HSPE1

Uniprot ID :

P61604

Immunogen :

A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10 (81-102aa VVLDDKDYFLFRDGDILGKYVD), different from the related rat and mouse sequences by one amino acid.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

10 kDa heat shock protein, mitochondrial

Synonyms :

10 kDa heat shock protein, mitochondrial; Hsp10; 10 kDa chaperonin; Chaperonin 10; CPN10; Early-pregnancy factor; EPF; HSPE1;

Protein Name :

10 kDa heat shock protein, mitochondrial(Hsp10)

Molecular Weight :

10932 MW

Protein Function :

Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.

Subcellular Localization :

Mitochondrion matrix.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

HSPE1 (heat shock 10kDa protein 1 (chaperonin 10)), also called CPN10, GROES, CHAPERONIN 10 HOMOLOG, cpn10 HOMOLOG or HSP10, is a protein that in humans is encoded by the HSPE1 gene. GroES is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The HSP10 gene consists of 4 exons. The HSPE1 gene is mapped to 2q33.1. The transcriptional activity of the promoter fragment in the HSP60 direction is approximately twice that in the HSP10 direction under normal growth conditions; upon heat shock, promoter activity in either direction increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the GroES overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.

Research Category :

Cancer, Chaperones, Heat Shock Proteins, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways And Processes, Protein Trafficking, Signal Transduction